EbbaBiolight
EbbaBiolight are optotracers for detection of bacteria & components of bacterial biofilm.
EbbaBiolight is available in five variants which label extracellular and intracellular bacterial amyloids as well as certain glucans produced as part of the extracellular matrix. Specifically, EbbaBiolight variants have been used as follows: EbbaBiolight 480 labels extracellular matrix produced by P. aeruginosa but not C. albicans in a mircofluidic device during mono- and co-culture. EbbaBiolight 630 and EbbaBiolight 680 label peptidoglycan and lipoteichoic acids in the cell wall of certain gram positives like Staphylococci and E. faecalis. EbbaBiolight 680 labels curli in the extracellular matrix produced by Salmonella as well as β-glucans and chitins in yeast- and hypal forms of C. albicans. It has been used to track pellicle formation in B. cenocepacia and label extracellular matrix components in Pseudomonas and E. coli.
All EbbaBiolight variants are exceptionally photostable and fluorogenic. When bound to a target, the optotracers can be imaged using fluorescence microscopy and spectral information can be acquired using a fluorescence spectrophotometer. As spectral information can give hints of the nature of the target, we recommend to acquire excitation and emission spectra whenever possible. All EbbaBiolight variants are intended for use in live-cultures. Fixation can lead to unspecific labelling of bacterial cells, regardless of them gram status. If fixation needs to be performed, we recommend to apply fixation after labelling.
EbbaBiolight variant work in a wide range of salt and pH conditions. When the pH is altered during the experiment, pH controls should be included. EbbaBiolight can be used with fluorescence plate readers, fluorescence microscopes and confocal laser scanning microscopes, fluorescence life time imaging, fluorescence cytometry, Total internal reflection fluorescence (TIRF) microscopy and Multiphoton microscopy.
Store your EbbaBiolight product in the fridge and use the opened container within 12 months. EbbaBiolight is for research use only and is not for resale.
EbbaBiolight Mix&Try is our recommended option for starting out with using EbbaBiolight. It contains 10 µL of each variant. Testing each variant in the will allow you to determine which one of our optotracers is best suited for your experiment.
All EbbaBiolight variants label extracellular and intracellular bacterial amyloids as well as certain glucans produced as part of the extracellular matrix. Specifically, the optotracers have been used as follows: EbbaBiolight 480 labeling extracellular matrix produced by P. aeruginosa but not C. albicans in a mircofluidic device during mono- and co-culture. EbbaBiolight 630 labeling peptidoglycan and lipoteichoic acids in the cell wall of certain gram positives like Staphylococci and E. faecalis. EbbaBiolight 680 labeling curli in the extracellular matrix produced by Salmonella as well as β-glucans, chitin and intracellular amyloid aggregates in yeast- and hypal forms of C. albicans. Furthermore, it has been used to track pellicle biofilm formation in B. cenocepacia. Contact us to learn more about EbbaBiolight applications.
All EbbaBiolight variants are exceptionally photostable and fluorogenic. When bound to a target, the optotracers can be imaged using fluorescence microscopy and spectral information can be acquired using a fluorescence spectrophotometer. As spectral information can give hints of the nature of the target, we recommend to acquire excitation and emission spectra whenever possible. Use recommended filter sets as well as excitation- and emission wavelengths according to the following table.
| Exmax | Emmax | Recommended filter-sets | |
|---|---|---|---|
| EbbaBiolight 480 | 420 nm | 480 nm | DAPI |
| EbbaBiolight 520 | 460 nm | 520 nm | FITC, GFP |
| EbbaBiolight 540 | 480 nm | 540 nm | FITC, GFP, YFP |
| EbbaBiolight 630 | 520 nm | 630 nm | PI, Cy3, TxRed, mCherry, Cy3.5 |
| EbbaBiolight 680 | 530 nm | 680 nm | PI, mCherry, Cy3.5 |
EbbaBiolight 680 is our red optotracer for labeling extracellular and intracellular bacterial amyloids as well as certain glucans produced as part of the extracellular matrix. Specifically, EbbaBiolight 680 labels curli in the extracellular matrix produced by Salmonella as well as β-glucans and chitins in yeast- and hyphal forms of C. albicans. Furthermore, it has been used to track pellicle biofilm formation by B. cenocepacia. Contact us to learn more about EbbaBiolight applications.
As all our optotracers, EbbaBiolight 680 is exceptionally photostable and fluorogenic. When bound to a target, EbbaBiolight 680 can be imaged using fluorescence microscopy and spectral information can be acquired using a fluorescence spectrophotometer. As spectral information can give hints of the nature of the target, we recommend to acquire excitation and emission spectra whenever possible. Use recommended filter sets as well as excitation- and emission wavelengths according to the table below.
| Exmax | Emmax | Recommended filter-sets | |
|---|---|---|---|
| EbbaBiolight 680 | 530 nm | 680 nm | PI, mCherry, Cy3.5 |
EbbaBiolight 680 is available in four different formulations (See volumes and prices in the drop-down list below):
- Aqueous: 1 mg/ml solution in ultrapure water. The product should be diluted 1:1000 before use. To prevent evaporation of the aqueous solvent, close the container carefully after use, spin down liquid and use up small volumes quickly.
- DMSO: 1 mg/ml solution in DMSO to prevent solvent evaporation. The product should be diluted 1:1000 before use. For use in live-cells, sometimes 1:500 is necessary due to uptake limitations.
- Solid: 1 mg solid lyophilised in a sterile injection bottle. We recommend dilution to 4 mg/ml in physiological saline followed by intravenous injection with a total dose of 5 mg/KG.
- Drop&Shine: 5 ml ready-to-use product in mounting medium. Ideal for use in tissue sections. Add a some Drop&Shine and mount your slide to detect biofilms within minutes.
EbbaBiolight 630 is our orange optotracer for labeling extracellular and intracellular bacterial amyloids as well as certain glucans produced as part of the extracellular matrix. Specifically, EbbaBiolight 630 labels peptidoglycan and lipoteichoic acids in the cell wall of certain gram positives like Staphylococci and E. faecalis. Contact us to learn more about EbbaBiolight applications.
As all our optotracers, EbbaBiolight 630 is exceptionally photostable and fluorogenic. When bound to a target, EbbaBiolight 630 can be imaged using fluorescence microscopy and spectral information can be acquired using a fluorescence spectrophotometer. As spectral information can give hints of the nature of the target, we recommend to acquire excitation and emission spectra whenever possible. Use recommended filter sets as well as excitation- and emission wavelengths according to the table below.
| Exmax | Emmax | Recommended filter-sets | |
|---|---|---|---|
| EbbaBiolight 630 | 520 nm | 630 nm | PI, Cy3, TxRed, mCherry, Cy3.5 |
EbbaBiolight 630 is available as a 1 mg/ml solution in volumes ranging from 10 - 200 µL (see volumes and prices in the list below). The product should be diluted 1:1000 before use. For use in live-cells, sometimes 1:500 is necessary due to uptake limitations. To prevent evaporation of the aqueous solvent, close the container carefully after use, spin down liquid and use up small volumes quickly.
EbbaBiolight 540 is our yellow optotracer for labeling extracellular and intracellular bacterial amyloids as well as certain glucans produced as part of the extracellular matrix. Contact us to learn more about EbbaBiolight applications.
As all our optotracers, EbbaBiolight 540 is exceptionally photostable and fluorogenic. When bound to a target, EbbaBiolight 540 can be imaged using fluorescence microscopy and spectral information can be acquired using a fluorescence spectrophotometer. As spectral information can give hints of the nature of the target, we recommend to acquire excitation and emission spectra whenever possible. Use recommended filter sets as well as excitation- and emission wavelengths according to the table below.
| Exmax | Emmax | Recommended filter-sets | |
|---|---|---|---|
| EbbaBiolight 540 | 480 nm | 540 nm | FITC, GFP, YFP |
EbbaBiolight 540 is available as a 1 mg/ml solution in volumes ranging from 10 - 200 µL (see volumes and prices in the list below). The product should be diluted 1:1000 before use. For use in live-cells, sometimes 1:500 is necessary due to uptake limitations. To prevent evaporation of the aqueous solvent, close the container carefully after use, spin down liquid and use up small volumes quickly.
EbbaBiolight 520 is our green optotracer for labeling extracellular and intracellular bacterial amyloids as well as certain glucans produced as part of the extracellular matrix. Contact us to learn more about EbbaBiolight applications.
As all our optotracers, EbbaBiolight 520 is exceptionally photostable and fluorogenic. When bound to a target, EbbaBiolight 520 can be imaged using fluorescence microscopy and spectral information can be acquired using a fluorescence spectrophotometer. As spectral information can give hints of the nature of the target, we recommend to acquire excitation and emission spectra whenever possible. Use recommended filter sets as well as excitation- and emission wavelengths according to the table below.
| Exmax | Emmax | Recommended filter-sets | |
|---|---|---|---|
| EbbaBiolight 520 | 460 nm | 520 nm | FITC, GFP |
EbbaBiolight 520 is available as a 1 mg/ml solution in volumes ranging from 10 - 200 µL (see volumes and prices in the list below). The product should be diluted 1:1000 before use. For use in live-cells, sometimes 1:500 is necessary due to uptake limitations. To prevent evaporation of the aqueous solvent, close the container carefully after use, spin down liquid and use up small volumes quickly.
EbbaBiolight 480 is our blue optotracer for labeling extracellular and intracellular bacterial amyloids as well as certain glucans produced as part of the extracellular matrix. Specifically, EbbaBiolight 480 has been used to label extracellular matrix produced by P. aeruginosa but not C. albicans in a mircofluidic device during mono- and co-culture. Contact us to learn more about EbbaBiolight applications.
As all our optotracers, EbbaBiolight 480 is exceptionally photostable and fluorogenic. When bound to a target, EbbaBiolight 680 can be imaged using fluorescence microscopy and spectral information can be acquired using a fluorescence spectrophotometer. As spectral information can give hints of the nature of the target, we recommend to acquire excitation and emission spectra whenever possible. Use recommended filter sets as well as excitation- and emission wavelengths according to the table below.
| Exmax | Emmax | Recommended filter-sets | |
|---|---|---|---|
| EbbaBiolight 480 | 420 nm | 480 nm | DAPI |
EbbaBiolight 480 is available as a 1 mg/ml solution in ultrapure water (Aqueous) with volumes ranging from 10 - 200 µL (see volumes and prices in the list below). The product should be diluted 1:1000 before use. For use in live-cells, sometimes 1:500 is necessary due to uptake limitations. To prevent evaporation of the aqueous solvent, close the container carefully after use, spin down liquid and use up small volumes quickly.
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